Inverted Fluorescence Microscope
Available magnifications: 10X, 20X, 40X and 63X air objectives, 100X oil immersion POL objective.
Camera: Leica DFC365 FX.
Light source: Halogen light source (12 V, 100 W)
Filter cubes: Leica filter cubes, A (UV), I3 (blue) and N2.1(green).
Software: Leica AF6000
Leica DMI3000 B is an inverted microscope and can be used for fluorescence imaging of materials and biological samples. This inverted fluorescence microscope is combined with phase contrast microscopy and dark field imaging at 10X, 20X magnification which can be further used for analytical confirmation of nano-scale materials and their interactions with biologicals or other composite materials. Three different filter cubes A (UV), I3 (blue) and N2.1 (green) provide excitation from UV-green region and can be used for excitation of multiple dyes. Leica AF6000 software with time lapse module can be used for imaging of samples in dynamic environment. It supports research in multiple areas such as fluorescent material's characterization bio and-labelling. Imaging analysis method can be used for fluorescently labelled components in live cells and nano-materials.
The Verios G4 UC features through-the-lens (TLD) SE and BSE detection specifically designed for high-resolution imaging at both high and low kVs, as well as an Everhart-Thornley SE detector for conventional SE detection. The in-lens SE detector features a technique where the energy range of the detected secondary electrons can be selected. The sample stage can be rotated 360 o and tiled to – 60 o making the system very versatile. Scan rate for the system ranges from 50 ms all the way upto 50 s.
Leica DM IL LED is an inverted microscope equipped with phase shift rings and gray filters for phase contrast microscopy. Although phase changes in the light transmitted through samples are invisible, they can be made visible when they are converted in to brightness variations and provide improved contrast in the image