qtrap

3200 QTRAP LC/MS/MS

Instrument specification:

The 3200 Q TRAP LC/MS/MS System is a high performance hybrid triple quadrupole linear ion trap LC/MS/MS mass spectrometer. The system includes: instrument, Turbo V source with TurboIonSpray and Atmospheric Pressure Chemical Ionization probes. Scans in a mass range of m/z 5-1700 in quadrupole mode and m/z 50-1700 in LIT mode.

About the Instrument:

The system provides excellent sensitivity, superior dynamic range, and reliability across a range of identification and quantitation applications. The system is best suited for quantitative analysis of small molecules, quantitative analysis of small molecules, proteins and peptides. The application involves measurement of specific molecular weight compounds such as drug or metabolite in a liquid sample, and their resulting fragment ions for determining the exact quantity of the sample of interest. The instrument can determine molecular weights and sequences of protein and peptide samples.

Theory of operation:

The 3200 Q TRAP mass spectrometer has a series of quadrupole filters(Q0-Q3) that transmit ions according to their m/z. Ions entering the ion path are first focused by Q0 before passing to Q1. Q1 is a filtering quadrupole that sorts the ions before they enter Q2, a Linac collision cell in which ions can broken into fragments by collisions with gas molecules. This technique allows for the design of experiments that measure the m/z of product ions to determine the composition of the parent ions. After passing through Q2, the ions enter Q3 for additional filtering, and then enter the detector. In the detector, the ions create a current that is converted into a voltage pulse. The voltage pulses leaving the detector are directionally proportional to quantity of ions entering the detector. The instrument monitors these voltage pulses and converts the information into a signal. The signal represents the ion intensity for a particular m/z and the instrument displays this information as a mass spectrum.

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