Centre of Excellence on Molecular Materials and Functions is one of the clusters of the Indian Institute of Technology Madras (IITM), as one of the ‘Institute of Eminence,’ launched by the Government of India to empower Higher Educational Institutions and help them become world-class teaching and research institutions.
Our vision is to build a sustainable center with global visibility on molecular matter, focusing on atomically precise clusters and gas hydrates. Seed, nurture, and expand cutting-edge science and technology in respective areas, collectively with the best people across the world, with the involvement of the next generation.
We aim to create a globally recognized Centre focusing on the synthesis and study of molecular materials, where the fundamental building blocks are molecules, rather than atoms. They hold considerable academic and technological promise as they offer tunable properties that are relevant for applications. In order to leverage the expertise of the participating groups in a synergistic fashion, we aim to initially specialize in two exciting and important families of molecular materials: those assembled from metallic clusters and those assembled from gas hydrate cages. However, as additional capabilities are established, we will also expand our activities into other kinds of molecular materials, including supramolecular polymers, energy-harvesting assemblies, molecular metals, and molecular magnets. Important strides have already been made in the study of molecular forms consisting of atomically precise metal clusters containing tens to hundreds of atoms; the participating groups have been among the leaders in this area.
The challenge now is to fill in the ‘gap’ between molecules and bulk materials, by achieving clusters of hundreds of kilodaltons and eventually megadaltons of mass. We will synthesize such systems, using novel methods and approaches. By studying the chemical, physical, and mechanical properties of these cluster-assembled solids, it is expected that we will achieve breakthrough results in reactivity, catalysis, electrical transport, magnetism, energy storage, and dissipation. In gas hydrates, our principal effort will be to use the hydrate cages as new molecular containers in which cryogenic chemistry can occur. Elementary processes happening in hydrate cages will be examined with in-situ spectroscopy. These will have immense relevance to molecular evolution in space as well as the utilization of hydrates for gas storage and CO2 sequestration. Alongside the experimental investigations, we will carry out theoretical studies that will shed light on the underlying phenomena and help optimize the properties of these molecular materials. In this way, a new understanding of materials and phenomena will be gained.
To maximize technological relevance, industrial linkages will be strengthened for local and national development. The outstanding team of collaborators, partners, and advisors that has been assembled will guarantee maximum international visibility and impact and further raise the profile of the Institute. Advanced facilities and resources will be created, and next-generation leaders will be trained with greater national and international participation, placing the larger objectives of the IoE as paramount. Thus, this proposal promotes local excellence while partnering with the best in the world.
Refeyn TwoMP mass photometer is used to accurately measure the mass of single particles or biomolecules, using a light scattering approach. It can accurately determine the mass of proteins as small as 20kDa. Using this approach, proteins, single particles, and other biomolecules can be characterized to study their function, interactions, oligomerization, and macromolecular assembly. This technique helps to assess sample integrity and homogeneity, in a facile and label-free method. As the instrument can gather data over time, it enables capturing the dynamic behavior of molecules or particles.
Mass photometry is quantitative mass imaging of single macromolecules in their true native state. It uses a light-scattering approach which allows for rapid measurement of the mass of individual proteins or particles. This technique provides high resolution, enabling the detection of low-abundance species, and has a wide working mass range. Using this tool, reliable measurement of molecular mass in the range of 30 kDa to 5 MDa is possible.
Notably, the mass photometry resolution varies across the mass range and can be affected by the composition and quality of a sample.
It is operable in a wide range of buffers. It can measure proteins on lipid bilayers and membrane-mimetic systems as well. For the TwoMP mass photometer, at the lower end of the mass range, the resolution is ± 25 kDa for a measurement of a 66 kDa biomolecule (defined as the Full Width of the peak at its Half Maximum value, FWHM). At the higher end of the mass range, for example, 660 kDa, the resolution is ± 60 kDa FWHM.
Key features of Refeyn TwoMP mass photometer
Mass range: 30 kDa – 5 MDa
Mass precision: ±2% or lesser
Mass error: ±5% or lesser (single measurement)
Resolution (FWHM): 25 kDa @ 66 kDa and 60 kDa @ 660 kDa
Concentration range: 100 pM – 100 nM
Sensitivity: << 1 ng of protein
Wavelength: 488 nm
Field of view: 4 x 11 µm (@ 500 Hz) up to 12 x 17 µm (@ 135 Hz)
Pixel size: 12 nm
Theory of operation
The mass photometry, a tool for “watching protein’s weight,” carefully measures light scattering and provides information on the mass of macromolecules and complexes. Unlike other light-based techniques, the mass photometry signal measured is directly correlated with the true molecular mass, enabling measurement of the mass of molecules in the range of 30 kDa to 5 MDa. The unlabeled single particles are adsorbed from the solution to a glass surface, and light is incident on it. The light scattered by a single particle at the glass-water interface is measured with potential sensitivity to obtain accurate mass. Nanoparticle aggregates, single particles, nanostructures. vesicles, polymers, biomolecules in their native state, such as nucleic acids, and viruses can be measured at a high resolution.
D8 Venture SC-XRD
Bruker Single Crystal X-ray Diffractometer (SCXRD), D8 Venture with Photon III C14 detector, is used to determine three dimensional crystal structures. It is equipped with two micro-focus X-ray sources (Cu and Mo radiation) providing a choice of two wavelengths, for measuring patterns in very small crystals. Powder diffraction of capillary samples can also be obtained. Grazing incidence measurements can also be made. Different cooling systems are available, enabling low temperatures such as 100 K.
Bruker SCXRD D8 Venture provides a high intense strongest X-ray source, with highly reliable largest X-ray detectors. With more room for rotating anode, liquid metal jet, and dual wavelength solutions, this instrument provides high versatility and flexibility to the user. The IμS Microfocus Sources yield Mo 0.71 Å and Cu 1.54 Å. The Mo radiation helps in high throughput and electron density measurements, and the Cu radiation provides absolute configuration determination of lightweight molecules.
Key features of D8 Venture SCXRD
Flux density: 6 × 10¹¹ X-rays/mm2s
X-ray sources: IµS 3.0 and IµS DIAMOND microfocus, METALJET liquid metal
Active area: up to 280 cm2
Anode: Microfocus TXS Rotating Anode
Goniometer: FIXED-CHI or KAPPA, for completeness and multiplicity
Software and data processing: APEX5 and PROTEUM3
Low temperature range: Cryostream (liquid nitrogen) and COBRA (non-liquid nitrogen)
Theory of operation
A crystal has a pattern of unit cells repeated in a three-dimensional lattice. Upon X-rays incident on the crystal lattice, it behaves like a diffraction grating, producing a diffraction pattern. A beam of parallel monochromatic X-rays of a specific wavelength is used to obtain this diffraction. The analysis of this diffraction pattern leads to the structure and shape of the lattice units. With the phase angles and a good fit between experimental and calculated diffraction patterns.
Prospective researchers or students can engage with us by writing to …..@….. with their resume and research proposal (if any).